|
Figure 4.
Figure 4. Ribbon diagrams and molecular surface representations
of CheW. a, Ribbon diagrams of the minimized average
structure of CheW. The two views are rotated 90° about the
x-axis. The order of the secondary structural elements is
indicated. Positions of absolutely conserved residues are shown
as balls; Gly 51, in red; and Arg 56, Gly 57, Gly 92 and Gly
124, in green. b, Chemical shift perturbations in CheW due to
binding of CheA^354 -671, mapped to a molecular surface
representation of CheW calculated from its minimized average
structure. Color ramp from white to red indicates 0 Hz to 150 Hz
change. Gray indicates no available data. c, Location of Tsr
repressor mutations (green) mapped onto the surface of CheW. d,
Combined map (a + b) of CheA interaction sites and repressor
mutations mapped to molecular surface of CheW. Overlap between
the two sites is shown in yellow. Surface representations were
created with GRASP34, after removal of the N-terminal nine amino
acids for clarity.
|
