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Figure 4.
Figure 4. A Close-Up View of the Interactions between the
Phosphorylated C Terminus from One Monomer and the Loop-Strand
Pocket of the Adjacent Monomer(A) An electron density map of the
phosphorylated C terminus. The 2F[o]-F[c] map (omit map), shown
in pink, was contoured at 1.5σ and was calculated by simulated
annealing using CNS (Brunger et al., 1998) with the omission of
the C-terminal five residues. The backbone as well as the side
chains of four residues are shown in yellow.(B) An overall view
of the interactions. The C terminus is shown as a yellow coil,
while its binding partner is represented as a transparent
surface with backbones in pink. The side chains of the last five
residues in the C terminus (CSSMS) and the basic residues in the
loop-strand pocket are shown.(C) A stereo view of hydrogen bond
networks. The two interacting monomers are shown in green and
blue, respectively. Their side chains are colored gold and
yellow. Hydrogen bonds among oxygen (red) and nitrogen (blue)
atoms and water molecules (red) are indicated by red dashed
lines.(D) A stereo view of the van der Waals contacts between
the phosphorylated C terminus from one monomer and the
loop-strand pocket of the adjacent monomer. The coloring scheme
is the same as in (C).
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