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Figure 4.
Figure 4. Top region of the C2-domain b-sandwich from PKCe.
(a) Superimposition of loops 1 and 3 from PKCe (magenta) with
the structurally equivalents loops CBR1 and CBR3 from the
Ca^2+-dependent C2 domain of PKCa (cyan). The two Ca ions
identified in the crystal structure of the C2 domain of PKCa are
depicted as green spheres. Three of the five aspartate residues
conserved in classical PKCs were replaced in PKCe by residues
Pro33, His85 and Ala87. The imidazole ring of His85 occupies the
position corresponding to the active Ca ion. (b) Superimposition
of loop 1 and loop 3 of PKCe onto the equivalent loops in PKCd.
The relative disposition of the loops and the orientation of the
side-chains within the pocket differs markedly between the two
novel PKCs.
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