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Figure 4.
Fig. 4. Comparison of the hypothetical hexamer model of
RuvB with the electron microscopic image. (a) Projection image
(Left) of negative stained RuvB complexed with a 30-bp DNA,
obtained by averaging 140 top views in our previous work (15).
The resolution of the averaged image was 30.0 Å. The top
views of the hexamer model (Center and Right) were constructed
by superimposing each ATPase domain of RuvB (AMPPNP form) (blue
region) onto the corresponding regions of HslU crystal structure
(25) and the NSF crystal structure (23), respectively. The
domains N, M, C, labeled residues, and the bound nucleotides are
represented with the same color code as defined in Fig. 1. (b)
Projection image (Left) of RuvB-DNA obtained by averaging 266
side views. This image of the single ring was taken from
one-half of the double ring, which encircles duplex DNA. The
resolution of the averaged image was 34.3 Å. Side view of
the hexamer model (Right). [Reproduced with permission from ref.
15 (Copyright 2000, Academic Press).
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