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Figure 4.
Fig. 4. Compound 4 prevents hair loss in a neonatal rat model
of CIA. Rat pups (13 days of age, actively growing hair) were
pretreated 4 hours and 2 hours (t =  4 and 2 hours)
with topical application of compound 4 (250 µg; 50
µl of 5 mg/ml DMSO) to the scalp, then injected with
etoposide. Etoposide induced total alopecia within 1 week of
administration. Hair was assessed and photographed on day 21.
The protective activity was schedule dependent. Two
applications, 4 hours and 2 hours before etoposide, were optimal
for protection. Post-treatment schedules were ineffective in
preventing hair loss. Shown are two animals from the untreated
group (- etoposide, upper left panel, animals 1 and 2); two
animals from DMSO-treated group (+ etoposide, upper right,
animals 3 and 4), and five animals from compound 4-treated group
(+ etoposide, lower panel, animals 5 to 9). Experiments were
repeated at least nine times with five rats per experimental
subgroup.
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