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Figure 4.
Figure 4. Structure of the affinity-improved Y0317 Fab
in complex with VEGF. A superposition of the structure
(Muller et al., 1998a) of wild-type humanized antibody
Fab-12 (gray) in complex with VEGF (gray) is shown
with that of Fab Y0317 (green) in complex with VEGF
(yellow). (a) Overall view of the complex, including one
Fab molecule bound to one dimer of VEGF (a second
Fab molecule is bound at left in the crystal) shows that
the binding site for both antibody variants centers on
the ``80's loop'' of VEGF. (b) A view of the four CDR
changes between Fab-12 and Y0317 Fab shows that the
new D28 and T100a side-chains do not directly contact
antigen. However, H31 and Y97 form new contacts.
(c) Interactions of H97 and an associated, buried water
molecule in the Fab-12 complex, compared with those of
Y97 in the Y0317 complex.
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