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Figure 3.
Figure 3. Schematic representation of the binding epitope
of VEGF for the humanized anti-VEGF antibody. Residues buried in
the interface as seen in the crystal structure are colored red;
residues marked with yellow display a greater than 20-fold
reduction in binding affinity when changed to alanine. For
comparison, and to allow discussion of the neutralizing effect
of the antibody, residues buried in the interface between VEGF
and domain 2 of the Flt-1 receptor [16] are colored blue, and
VEGF binding determinants for KDR [15] are in green. The
position of the twofold axis of the VEGF dimer is indicated by a
black ellipse.
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