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Figure 3.
Figure 3. Stereo views of buried surface areas and residues
of the FabE8-cyt c (a) paratope with 48 interface water
molecules, and (b) E8 epitope. Views are 90° fromFigure 1a.
Water molecules (green spheres) form a collar around the buried
surface areas of E8 antibody (pink mesh) and cyt c (blue mesh).
C^a traces of E8 V[L] are shown in white, V[H] in yellow and cyt
c in orange. Side-chains in the E8 paratope are displayed and
labelled with CDRs L1 and H1 in red, L2 and H2 in yellow and L3
and H3 in green. The E8 epitope consists of three major
discontiguous segments (33 to 39; 56 to 66 and 96 to 104) shown
with magenta side-chains and two minor sites composed of the
N-terminal acetyl group (Ac), Val3 (top) and Lys22 (left) (with
white side-chains and yellow labels) at the periphery. Arg38 and
Tyr74 (white side-chains and green labels) lie outside the E8
epitope. Oxygen and nitrogen atoms are shown as red and blue
spheres, respectively.
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