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Figure 3.
Figure 3. Reaction mechanism for a retaining
endo-β-1,4-xylanase. R is a number of xylose residues, HA is
the acid catalyst. The structures in brackets are possible
intermediates and R[1] is hydrogen or a number of xylose
residues. Intermediate (a) has the nucleophile stabilizing the
oxo-carbonium ion, whereas in (b) the covalent intermediate is
formed. Either of these intermediates could react with water and
be hydrolyzed or react with another xylo-oligosaccharide to
produce trans-glycosylation products. Figure 3. Reaction
mechanism for a retaining endo-β-1,4-xylanase. R is a number of
xylose residues, HA is the acid catalyst. The structures in
brackets are possible intermediates and R[1] is hydrogen or a
number of xylose residues. Intermediate (a) has the nucleophile
stabilizing the oxo-carbonium ion, whereas in (b) the covalent
intermediate is formed. Either of these intermediates could
react with water and be hydrolyzed or react with another
xylo-oligosaccharide to produce trans-glycosylation products.
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