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Figure 3.
(a) Binding of TAB2 NZF to Lys63-linked and linear (Lin) di-
and tetraubiquitin. (b) Close-up of the Lys63 linkage showing
the position of Met1 at 10.8 Å distance. (c) Binding of
TAB2 NZF to Lys63- and Lys48-linked di- and tetraubiquitin. (d)
Comparison of TAB2–Lys63-diubiquitin and hHR23A
UBA2–Lys48-diubiquitin (PDB 1ZO6 (ref. 8)). (e) Superposition
of the distal ubiquitin moieties (dark green) from d, with TAB2
shown. Because of the flexible isopeptide linker, the proximal
ubiquitin of Lys48-diubiquitin (light green) can rotate to adopt
a similar conformation as Lys63-diubiquitin on TAB2 (see also
Supplementary Fig. 6).
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