|
Figure 3.
Crystal structures of Dpo4 bound to N^2,N^2-Me[2]G-modified
DNA.A, superimpositions of DMG-1 (red), DMG-2 (molecule A,
cyan), and DMG-3 (molecule A, green) reveal overall similarity
in Dpo4 structure. B, representative electron density near the
active site of Dpo4 in the DMG-1 structure. The 3F[o][]− 2F[c]
map (gray mesh) is shown contoured to the 1σ level. The
F[o][]− F[c] difference maps are shown contoured to 3σ and
−3σ for positive (red mesh) and negative (green mesh)
density, respectively. The terminal C[dd] residue is flipped out
of base-stacking orientation. but the incoming dGTP forms a
Watson-Crick pair with the cytosine to the 5′-side of
N^2,N^2-Me[2]G (DMG).
|
