Figure 3.
(a) TRAF6 mutants defective in Ubc13 interaction failed to
rescue IL-1–induced TRAF6 autoubiquitination in
TRAF6-deficient MEFs. The indicated stable cells lines were
treated with IL-1 (1 ng ml^-1) for the indicated times and the
clarified lysates were immunoblotted with the indicated
antibodies. (b) TRAF6 mutants defective in Ubc13 interaction
failed to rescue IL-1–induced IKK activation and I B
phosphorylation in Traf6^-/- MEFs. IB, immunoblot; WT, wild type.
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
Nat Struct Biol
(2009,
16,
658-666)
copyright 2009.
B
phosphorylation in Traf6^-/- MEFs. IB, immunoblot; WT, wild type.