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Figure 3.
Crystal structures of EGFR 287–302 bound to the Fab
fragments. (A) Cartoons of Fab806 (Upper), with the light chain
(red), heavy chain (blue), bound peptide (yellow), and the
superposed EGFR[287–302] from EGFR (purple) and Fab175
(Lower), with the light chain (yellow), heavy chain (green),
bound peptide (lilac), and EGFR[287–302] (purple). (B)
Detailed stereoview of 175 Fab complex, viewed orthogonal to A,
looking into the antigen-binding site. Peptide backbones are
shown as cartoon and Cα traces and the interacting side chains
as sticks (same coloring as in A). Atoms are colored as follows:
O, red; N, blue; and S, orange; C is as for the main chain and
the side chain hydrogen bonds are dashed. Water molecules buried
upon complex formation are red spheres. (C) Superposition of
EGFR (turquoise surface with the 806 epitope displayed as a
purple Cα trace) with the Fab175:peptide complex showing
spatial overlap (same coloring as in B).
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