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Figure 3.
Figure 3. Cryo-EM Density Map of the MCM Hexamer
(A and
C) Surface rendering of the cryo-EM density map (displayed at
2σ) viewed from the top and the side, respectively. The side
view clearly shows the lateral holes, which are disrupted by an
isthmus of electron density.
(B and D) Fitting of the AAA+
domain from MkaMCM2 (red) and the N-terminal domain from MthMCM
(green, Fletcher et al., 2003). The AAA+ domain of MkaMCM2
matches well the dome-shaped electron density.
(E) A view
showing the fitting of two hybrid monomers (including the
N-terminal domain of MthMCM and the AAA+ domain of MkaMCM2). The
N-terminal domain of one subunit (highlighted in green)
communicates with the AAA+ domain of the next-neighboring
subunit (in red) through the interaction between the PS1BH of
the AAA+ domain (shown in yellow) and the β7-β8 loop of the
N-terminal domain of an adjacent subunit (in orange) consistent
with biochemical studies on MthMCM (Sakakibara et al., 2008).
(F) A close up of the same interaction.
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