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Figure 3.
Figure 3. ITC Characterization of Ca^2+/CaM-Ca[V]2.1 IQ
Domain Interactions
(A) 75 μM Ca^2+/N-lobe into 7.5 μM
Ca[V]2.1 IQ domain.
(B) 75 μM Ca^2+/C-lobe into 7.5 μM
Ca[V]2.1 IQ domain.
(C) 75 μM Ca^2+/N-lobe into 7.5 μM
Ca[V]2.1 IQ domain Y1974A (position (+3)).
(D) 75 μM
Ca^2+/C-lobe into 7.5 μM Ca[V]2.1 IQ domain Y1974A (position
(+3)).
(E) 250 μM Ca^2+/C-lobe into a solution of 25 μM
Ca[V]2.1 IQ domain and 33 μM Ca^2+/N-lobe.
(F) 250 μM
Ca^2+/C-lobe into a solution of 25 μM Ca[V]2.1 IQ domain I1965A
(position (−6)), and 33 μM Ca^2+/N-lobe.
(G) 250 μM
Ca^2+/C-lobe into a solution of 25 μM Ca[V]2.1 IQ domain M1969A
(position (−2)), and 33 μM Ca^2+/N-lobe.
(H) Comparison
Ca^2+/C-lobe binding isotherms to Ca^2+/N-lobe-Ca[V]2.1 IQ
domain complexes. ITC Panels show addition of 10 μl aliquots of
titrant to the target (top) and binding isotherms (bottom).
Cartoons depict the observed binding modes. Stars indicate
mutant peptides and site of mutation.
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