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Figure 3.
Fig. 3. Comparison of calcyphosine with CaM. (a) Evolutionary
relationship of calcyphosine with some of its nearest homologs.
The sequences of some typical Ca^2 +-binding proteins with four
EF-hand motifs were aligned using ClustalW, and the output tree
file was displayed as a dendrogram using the program TreeView
1.6.6. The proteins are human proteins, unless stated in the
dendrogram. (b) Superposition of calcyphosine and CaM (PDB code
1CLL) based on EF-hands 3 and 4. The color scheme is the same as
for Fig. 1a, with CaM shown in magenta. All figures were
prepared using PyMOL (http://www.pymol.org). (c) Sequence
alignment of calcyphosine with CaM. N-terminal additional
α-helix αA and C-terminal inserted α-helix αJ are shown in
blue boxes. Residues that correspond to the target recognition
in the C-domain of CaM are highlighted with cyan spheres.
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