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Figure 3.
The x-ray crystal structure of ePDZ-a in complex with the
ARVCF peptide. (A) Ribbon representations of the overall
structure. The cpPDZ and FN3 portions and the peptide are shown
in gray, cyan, and yellow, respectively. Missing residues for
the linker segment are indicated with dashed lines. (B) Clamping
of the peptide by ePDZ-a. Only the region in the dashed box in A
is shown. The surfaces originating from the cpPDZ and FN3
portions are shown in gray and cyan, respectively, and the
peptide is shown as a yellow stick model. (C) Interactions of
the FN3 loops with the cpPDZ/peptide complex. The three FN3
loops (BC, DE, and FG loops) are shown as sticks in blue, cyan,
and red, respectively. The surface of the PDZ portion is shown
in gray, and the peptide is shown as yellow spheres. In Lower,
the surfaces of the PDZ and peptide portions in contact with the
BC, DE, and FG loops (within 5 Å are shown in blue, cyan,
and red, respectively, and those in contact with both BC and FG
loops are in magenta. The peptide surfaces without FN3 contact
are shown in yellow, and the green line encloses the bound
peptide. (D) Superposition of wild-type PDZ (PDB entry 1MFG,
green) and cpPDZ (gray). The original and new termini are
indicated. The rmsd for the equivalent 97 Cα atoms was 0.54
Å. The structures are in the same orientation as in A.
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