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Figure 3.
FIGURE 3. The building blocks of the interface. The cyt
c[1] (A)-cyt c (B) interface is shown in open book view. The
heme groups (black) are encircled by the core interface (orange
area, interacting atoms as orange spheres). The variable
interface (yellow) surrounds the latter. Residues mediating
long-range electrostatic interactions (pink and blue for
negative and positive charges, respectively) form a semicircle
around the central hydrophobic contact site. C, the
semitransparent surface representation of cyt c[1] (gray) and
cyt c (green) shows the position of these long-range
electrostatic interactions in the high resolution structure
(Table 1) as highlighted in blue-green and pink (positive and
negative charges, respectively). Heme groups are shown in black.
A high number of water molecules (cyan spheres) are bound at the
interface of cyt c[1] (D) but not of cyt c (E). Only two of
these water molecules form hydrogen bonds to both cyt c[1] and
cyt c (green spheres). F, interface water molecules bound to cyt
c[1] colored in cyan and green (as in D) are superimposed with
all surface water molecules that are present at the other cyt
c[1] with the non-occupied cyt c binding site (dark blue
spheres). Surface water molecules up to a distance of 3.5
Å are included.
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