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Figure 3.
Fig. 3. The TRF2-TIN2 interaction. (A) Co-IP of TIN2 with
cotransfected wild-type and mutant TRF2. (B) Far-Western
analysis of the TIN2 binding region of TRF2 (FL, full-length;
TRF2-  B, TRF2- 1–42). (C)
Superposition of the TIN2[TBM] binding sites in the
TRF1[TRFH]-TIN2[TBM] and TRF2[TRFH]-TIN2[TBM] complexes.
TRF1[TRFH] and TRF2[TRFH] are in green and cyan, respectively.
The TIN2[TBM] peptides bound to TRF1[TRFH] and TRF2[TRFH]are
shown in stick model format and in yellow and magenta,
respectively. (D) TIN2-F258 interacts less efficiently with TRF2
than with TRF1. The F258 binding surfaces of TRF1[TRFH] (top
panel) and TRF2[TRFH] (bottom panel) are shown in magenta
(hydrophobic patch) and blue (hydrophilic patch). The rest of
TRF1[TRFH] and TRF2[TRFH] is in green and cyan, respectively.
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