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Figure 3.
Figure 3. The binding of the anti-inducer, ONPF, the
repressor in the absence and the presence of the operator. (a)
In the presence of DNA, the anti-inducer forms a ternary complex
with the repressor primarily by establishing hydrogen bonds
between the O2 and O3 hydroxyls of the fucoside and residues
R197, N246 and D274 of the repressor and the nitrophenyl group
hydrogen bonds to N146. (b) In absence of DNA, the anti-inducer
is also bound to the repressor by hydrogen bonding to the
fucoside but the nitrophenyl group does not appear to be ordered
or adopt the same conformation.
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