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Figure 3.
Fig. 3. Unfolding forces. (a) Unfolding forces of TNfn3
(blue), FNoTNc (red), and FNfn10 (black). FNoTNc unfolds at the
same force as TNfn3. Only FNfn10 shows double peaks, indicting
the presence of an unfolding intermediate. These traces were
collected at a retraction speed of 1,000 nm/s. (b) Histograms of
unfolding forces of TNfn3 (blue), FNoTNc (red), and FNfn10
(black) at a retraction speed of 1,000 nm/s. The modal unfolding
forces are 127 ± 3 pN (n = 305), 125 ± 3 pN (n =
332), and 104 ± 5 pN (n = 172), respectively [see also
supporting information (SI) Fig. 5].
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