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Figure 3.
Figure 3 (A, B) Representative structures of the two different
^1-2FNIII forms present in the structure calculation. Occupancy
of form A in the final ensemble was 78%, whereas that of form B
was 22%. Side chains for two residues, K669 and D767, involved
in an electrostatic interaction in form A but not form B, are
shown. D767 is also involved in electrostatic interactions with
K672 in population A, and participates in an extended hydrogen
bond and electrostatics network on the ^1FNIII binding interface
of ^2FNIII. The interdomain linker is schematically represented
as a dashed line. (C, D) ^1-2FNIII structure. Shown here are:
the final 39-structure ensemble of ^1-2FNIII structures
displaying (C) or omitting (D) the flexible ^1FNIII–^2FNIII
linker. Secondary structure elements are colored cyan and purple
for ^1FNIII and ^2FNIII, respectively.
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