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Figure 3.
Fig. 3. Raman spectra of SOR crystals. After reaction with
H[2]O[2], the E114A SOR mutant reveals bands at  567
cm^–1 and 838 cm^–1, which
are isotopically shifted to 563 cm^–1 and
802 cm^–1 in the
presence of H ^18[2]O[2] (vertical gray lines). Similar Raman
bands and ^18O isotopic shifts are observed in solution
experiments (fig. S2). E114A-SOR in the native reduced form does
not exhibit these bands; neither do crystals oxidized by
hexachloroiridate(IV). The peaks at 567 cm^–1 and
838 cm^–1 are not
substantially affected by exposure to an x-ray dose of 3 x 10^5
Gy, which is about the same dose as used for data collection.
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