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Figure 3.
Figure 3. Conserved Elements of PilT Fold
(A) The NTD of
PilT (colored as in Figure 2A) resembles the well-known PAS
domain (gray, represented by the circadian clock protein Period;
Yildiz et al., 2005). Noncanonical PAS elements (PilT αA and
loops within Period) are removed for clarity.
(B) The core
ATPase subdomain of PilT (green) is readily superimposable upon
RecA (Story and Steitz, 1992) (gray). In this view, the
least-squares calculation is over P-loop residues only. Type
II/IV secretion ATPase family motifs Walker A (blue), Asp box
(lime green), Walker B (magenta), and His box (orange) neighbor
the bound nucleotide.
(C) Isolated, magnified view of the
four sequence motifs described in (B), with ATP and signature
invariant residues Lys149, Glu176, Glu217, and His242 depicted
(blue, green, magenta, and orange, respectively).
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