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Figure 3.
Figure 3. Interactions between UbVME and M48^USP
(A)
Stereo view of the extended C terminus and attached VME moiety
of Ub in the M48^USP active-site cleft. M48^USP and UbVME are in
gray and green, respectively. Nitrogen atoms are shown in blue,
and oxygen atoms in red. Dashed lines indicate hydrogen bonds.
Note that the Ub C terminus features an extended β conformation
and is extensively coordinated by several hydrogen bonds to
M48^USP residues. V140 and Y76 of M48^USP are in van der Waals
contact and form a canopy over the active site.
(B) Stereo
view of the interactions between the Ub core (green) and M48^USP
(gray) or its β hairpin (orange). The gray transparent M48^USP
surface highlights the shape complementarity of the interface,
which is mainly lined by hydrophobic residues.
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