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Figure 3.
Figure 3. Different CDR1–CDR3 contacts in Ancestral and
PBLA8. (a) unliganded Ancestral, (b) unliganded PBLA8, (c)
liganded Ancestral, (d) liganded PBLA8. Residue 28 is an Asn in
the Ancestral structure (a) and an Arg in PBLA8 (b). PBLA8 Arg28
forms a salt-bridge with Asp93 that may serve to stabilize
CDR3; this interaction is absent in the Ancestral structure.
The PBLA8 Arg28-Asp93 salt-bridge must be broken to bind
lysozyme (d) where Asp93 forms a salt-bridge with lysozyme
residue ArgL112. Figure 3. Different CDR1–CDR3 contacts in
Ancestral and PBLA8. (a) unliganded Ancestral, (b) unliganded
PBLA8, (c) liganded Ancestral, (d) liganded PBLA8. Residue 28 is
an Asn in the Ancestral structure (a) and an Arg in PBLA8 (b).
PBLA8 Arg28 forms a salt-bridge with Asp93 that may serve to
stabilize CDR3; this interaction is absent in the Ancestral
structure. The PBLA8 Arg28-Asp93 salt-bridge must be broken to
bind lysozyme (d) where Asp93 forms a salt-bridge with lysozyme
residue ArgL112.
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