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Figure 3.
Figure 3. Structures of the Peroxisomal Import Receptor
Pex5p(C) in the Presence and in the Absence of the Cargo mSCP2
Color coding for Pex5p(C) is as follows: TPR1-TPR3, cyan;
TPR4, green; TPR5-TPR7, blue; 7C loop, connecting TPR7 and the
C-terminal helical bundle, red; and C terminus, maroon. Color
coding for mSCP2 is as follows: core domain, yellow; and C
terminus including PTS1 motif, orange. The orientation of the
receptor in (A) and (C) is identical. The ribbon of the
Pex5p(C)/mSCP2 complex in (B) has been rotated by 60° around
a horizontal axis within the paper plane with respect to the
orientation in (A), to illustrate the mode of mSCP2
binding to the receptor. (D) Superimposed Pex5p(C) receptor
structures in the presence and in the absence of mSCP2. The
colors of the trace of the cargo-loaded conformation are as in
(A)–(C), except that the conformational hinge regions are
colored in orange. The trace of the apo-Pex5p(C) structure is in
gray, except for the 7C loop, which is colored in faint red. The
coordinates of TPR segments 1–4 were used for structural
superposition using the program SSM (Krissinel and Henrick,
2004) (rmsd = 0.78 Å for 164 common residues). The largest
structural deviations of up to 8 Å are observed at the 7C
loop and adjacent regions and are indicated by a red arrow.
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