|
Figure 3.
Fig. 3. HhN binds to a cleft on IhogFn1. (A)
Semitransparent molecular surface of the HhN/IhogFn1–2 complex
superimposed on a ribbon diagram of the molecules. HhN is
colored yellow, IhogFn1 is green, and IhogFn2 is light blue. The
four residues that when mutated lead to loss of heparin binding
are colored dark blue. (B) Electrostatic potential surface of
the HhN/IhogFn1–2 complex, shown in the same orientation as in
A. The black dotted line marks the boundary between HhN and
IhogFn1–2. The four residues that when mutated lead to loss of
heparin binding are outlined with white dashes. The color scale
is calibrated to –12 kT/e and +12 kT/e for red and blue,
respectively. (C) HhN/IhogFn1–2 interface. The four Hh
residues mutated in the HhN/Ihog-interface 1 mutant are
represented as balls and sticks, as are nearby IhogFn1–2
residues. Bridging waters are represented by red spheres and
hydrogen bonds are shown as dashed lines. (D) Effects of
HhN-interface mutations on HhN binding to Ihog-expressing
Drosophila cultured cells.
|
