Figure 3.
FIGURE 3. Structural comparison of MHCI binding sites
between LILRBs and LILRA5. A, center, the LILRB1 (light pink) of
the HLA-A2 complex model (4) (heavy chain; blue, 2-microglobulin; cyan)
is superimposed onto LILRA5 (red). Left and right, large
structural difference observed in D1 (right, orange dotted
circle around the C' strand) and D2 (right, blue dotted circle
around the DE loop). LILRB1 (light pink) and LILRB2 (light
green) are superimposed onto LILRA5 (red). B, the ribbon model
of CC' sheet of LILRA5 (red) is superimposed onto those (C
strand and 3[10] helices) of LILRB1 (light pink). Conserved
amino acids are shown in a ball model (left), and their colors
are the same as those in amino acid comparison among LILR
members (right). Key amino acids for structural changes are
colored, and this coloring is also applied in the labels in C.
The characters, h and s, on the sequence alignment indicate
helix and sheet structures, respectively. C, details of local
structures around the 3[10] helix of LILRB1 (left) and C' sheet
of LILRA5 (right), highlighted by the orange dotted circle in A.
The black dotted lines are shown in order for the main-chain
structures to be easily recognized. The hydrophobic core of
LILRB1 (left) and hydrophobic pocket of LILRA5 (right) are
indicated by the red dotted circles.
The above figure is reprinted
by permission from the ASBMB:
J Biol Chem
(2006,
281,
19536-19544)
copyright 2006.
2-microglobulin; cyan)
is superimposed onto LILRA5 (red). Left and right, large
structural difference observed in D1 (right, orange dotted
circle around the C' strand) and D2 (right, blue dotted circle
around the DE loop). LILRB1 (light pink) and LILRB2 (light
green) are superimposed onto LILRA5 (red). B, the ribbon model
of CC' sheet of LILRA5 (red) is superimposed onto those (C
strand and 3[10] helices) of LILRB1 (light pink). Conserved
amino acids are shown in a ball model (left), and their colors
are the same as those in amino acid comparison among LILR
members (right). Key amino acids for structural changes are
colored, and this coloring is also applied in the labels in C.
The characters, h and s, on the sequence alignment indicate
helix and sheet structures, respectively. C, details of local
structures around the 3[10] helix of LILRB1 (left) and C' sheet
of LILRA5 (right), highlighted by the orange dotted circle in A.
The black dotted lines are shown in order for the main-chain
structures to be easily recognized. The hydrophobic core of
LILRB1 (left) and hydrophobic pocket of LILRA5 (right) are
indicated by the red dotted circles.