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Figure 3.
Figure 3. The CheZ[200-214] peptide-CheY interface. Ribbon
representation of (a) the F432YZ[200-214] interface and (b) the
P2(1)2(1)2YZ[200-214] interface. The side-chains of key
contacting residues are illustrated as ball and stick models and
hydrophobic contacts are shown as green patches. (c) Relative
B-factors of CheZ[200-214] in the CheY-CheZ[200-214] structures.
The relative B-factor versus CheZ residue number plot in Click
to view the MathML source- [0?wchp=dGLbVlb-zSkzk]
-free F432YZ[200-214] is shown in cyan, that in Click to view
the MathML source- [0?wchp=dGLbVlb-zSkzk]
-bound F432YZ[200-214] in deep blue and that in Click to view
the MathML source- [0?wchp=dGLbVlb-zSkzk]
-bound P2(1)2(1)2YZ[200-214] in orange. B[residue] is the
overall B-factor for each residue and B[CheZ] is the overall
B-factor for all atoms of CheZ[200-214] included in the final
model. (d) Schematic representation of the CheY-CheZ[200-214]
contacts. The F432Z[200-214] primary sequence in cyan and the
P2(1)2(1)2Z[200-214] primary sequence in orange are shown on
either side of the C-terminal half of CheY, represented in
secondary structural elements. Participating residues are
highlighted. Hydrophobic contacts are illustrated as continuous
grey lines, salt bridges as broken black lines and hydrogen
bonds as continuous black lines. The Click to view the MathML
source- [0?wchp=dGLbVlb-zSkzk]
-free and Click to view the MathML source- [0?wchp=dGLbVlb-zSkzk]
-bound F432YZ[200-214] structures solved from crystals grown in
Tris (pH 8.4) are used as representatives of F432YZ[200-214]
structures in this Figure.
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