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Figure 3.
Fig. 3. The conserved PknB homodimer. (a) Superposition of
the two crystallographically independent homodimers from the
PknB-mitoxantrone complex (in red and green) with those observed
in the PknB-nucleotide complexes 1O6Y [3] (in blue) and 1MRU [4]
(in yellow). (b) Overall view of the PknB monomer (rotated
90° along the vertical axis with respect to the right
monomer in Fig. 3a), color-coded according to amino acid
conservation (red: highly conserved) in 39 PknB-like protein
sequences from 35 different bacterial species (Bacillus
anthracis, B. cereus, B. clausii, B. licheniformis, B. subtilis,
Bifidobacterium longum, Clostridium acetobutylicum, C.
perfringens, C. tetani, Corynebacterium diphtheriae, C.
efficiens, C. glutamicum, Enterococcus faecalis, Geobacillus
kaustophilus, Lactobacillus acidophilus, L. johnsonii, Listeria
monocytogenes, Mycobacterium avium, M. bovis, M. leprae, M.
tuberculosis, Nocardia farcinica, Nocardioides, Leifsonia xyli,
Oceanobacillus iheyensis, Propionibacterium acnes,
Staphylococcus haemolyticus, S. saprophyticus, Streptococcus
agalactiae, S. mutans, S. pyogenes, Streptomyces coelicolor,
Symbiobacterium thermophilum, Thermoanaerobacter tengcongensis,
Thermobifida fusca). (c) Comparison of the PknB and
RNA-dependent PKR dimer interfaces. The side-chain residues
belonging to the interfaces are shown (PknB color-coded as in
(b)).
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