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Figure 3.
Figure 3 Sequence variations or mutations in TRIM5  ,
Pyrin, and MID1. (A) Location on the primary structures. The
diagrams depict the primary structures of the three proteins and
the domains they possess. The locations of the sequence
variations or disease-causing mutations are marked on the
diagrams for TRIM5 :
pink triangles, locations of the significant sequence variation
and length polymorphism in primate TRIM5 proteins;
pink arrow, the substitution of R332P in human TRIM5 that
confers the ability to restrict HIV-1, for Pyrin: blue arrows,
the FMF-causing point mutations including three mutational hot
spots marked with an asterisk, and for MID1: white arrows, the
OS-causing frame shift or nonsense mutations; yellow arrows,
point mutations, insertion, or deletion of amino acids. (B)
Location of the corresponding residues of GUS on the tertiary
structure. The sequence variations or mutations in the three
proteins are mapped on the structure of the B30.2/SPRY domain of
GUS. The mutation sites are indicated by large C atom
spheres and labels shown in the same color of the arrows in (A).
The residues of GUS corresponding to the mutation points are in
the parentheses. The loop regions in pink correspond to the
locations of the length polymorphism in the primate TRIM5 proteins.
'Insertion' stands for the eight amino-acid insertional mutation
in MID1, and 'del' stands for deletion of a residue in Pyrin. A
schematic drawing of the  -sandwich
structure of GUS is shown to aid the recognition of surface A
and surface B.
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