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Figure 3.
Figure 3 Overall structure of TbRET2. (A) Ribbon presentation of
the molecule. The three domains are shown in green (NTD), yellow
(MD) and blue (CTD) (as in Figure 1). The UTP-Mg2+ at site A is
shown as ball-and-stick in a cleft between the NTD and the CTD.
(B-D) Electrostatic potential surface of TbRET2. The positive
and negative regions are colored in blue and red, respectively.
Nucleotide-binding sites are labeled in yellow. UTP/UMP and Mg2+
are shown as ball-and-stick. Notice the extended blue patch
across three domains that suggests an RNA binding region. (B)
Front view, UTP-binding site A with a UTP and nucleotide-binding
site B with a UMP molecule; (C) side view (90° upward rotation),
nucleotide-binding site B with a UMP molecule; (D) back view
(180° upward rotation), nucleotide-binding site C with a UMP
molecule. Figure generated by PYMOL (DeLano, 2002).
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