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Figure 3.
Figure 3. Superposition of PTP1B Structures
(A) The Ca
trace of PTP1B from the PTP1B-IRK complex is colored cyan, and
the superimposed Ca trace from the PTP1B-phosphopeptide
structure (Salmeen et al., 2000) (PDB code 1G1F) is colored
orange. The WPD loop (residues 179-184) in each PTP1B structure
is colored gray, and the catalytic loop (residues 215-218) is
colored red. The b2-b3 loop of IRK from the PTP1B-IRK structure
is colored yellow, and the IRK phosphopeptide from the 1G1F
structure is colored magenta. Select secondary structure
elements are labeled, and the N and C termini of the two PTP1B
structures are denoted by "N" and "C," respectively. The first
ordered residue in both structures is E2, while the last ordered
residue is M282 in the PTP1B-IRK structure and D298 in the 1G1F
structure. Where termini or secondary structure elements overlap
between the two PTP1B structures, the labels are colored black,
otherwise they are colored cyan (PTP1B-IRK) or orange (1G1F).
The black rectangle indicates the zoom area in (B).
(B)
Stereoview near the active site of PTP1B. Select side chains of
the two PTP1B structures, as well as the sulfate ions (labeled
S1, S2, and S3) in the PTP1B-IRK structure, are shown in
ball-and-stick representation. Carbon atoms are colored cyan
(PTP1B from PTP1B-IRK), orange (PTP1B from 1G1F), or magenta
(phosphopeptide from 1G1F), oxygen atoms are colored red,
nitrogen atoms are colored blue, phosphorus atoms are colored
black, and sulfur atoms are colored green.
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