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Figure 3.
Figure 3 Folding and interaction of the two isolated, partial PH
domain fragments. (A) SDS-PAGE showing the purification of the
joined PH[N]-'C'-PH[C] domain and cleavage of the domain into
PH[N] and PH[C] fragments. (B) Overlay plot of the 1H, 15N HSQC
spectra of the joined PH[N]-'C'-PH[C] (black) and its protease
cleaved form (red). 1H, 15N HSQC spectra of the isolated PH[N]
(C) and PH[C] (D) fragments. (E) Overlay plot of the 1H, 15N
HSQC spectra of the joined PH[N]-'C'-PH[C] (black) and the 1:1
mixture of the two halves of the split PH domain (red).
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