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Figure 3.
Figure 3
(a) Polypeptide tracings of the VH and VL domains of the HuAF2 Fab (PDB code [187]1b4j )
in red, superimposed on those of the murine domains in the mouse-human chimeric ChiAF2 Fab
(PDB code [188]1b2w ) in white. CDRs in the HuAF2 have amino-acid sequences excerpted from
those in the original murine AF2 antibody. (b) Backbone tracings of HuZAF V domains (cyan)
overlaid on those of ChiAF2 (Maloney et al., 1997[189] [Maloney, D. G., Grillo-Lopez, A.
J., White, C. A., Bodkin, D., Schilder, R. J., Neidhart, J. A., Janakiraman, N., Foon, K.
A., Liles, T. M., Dallaire, B. K., Wey, K., Royston, I., Davis, T. & Levy, R. (1997).
Blood, 90, 2188-2195.]-[190][bluearr.gif] ). In HuZAF, the substitution of two acceptor
framework residues with mouse residues, Val11 by leucine and Arg38 by lysine,
significantly improves the structural mimicry relative to ChiAF2.
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