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Figure 4.
Figure 4. Structure-Based Mutagenesis of the TalinAt left,
binding of talin mutants to full-length β3 tail. Recombinant
F2+F3 containing the indicated mutation was incubated with the
integrin β3 cytoplasmic domain model protein; following
washing, the bound protein was detected by SDS-PAGE. Note the
absence of binding to β3(Y747A) and αIIb cytoplasmic domain
model proteins and the equal loading (rightmost column) of each
mutant. The bottom row depicts the equal loading of each model
tail protein as judged by SDS-PAGE. At right, residues mutated
in this study are mapped onto the surface of the talin F3
domain, with hydrophobic residues colored yellow. The K357A
mutation (green) does not affect binding, while alanine
substitution of R358, W359, and I396 (red) reduce binding.
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