Figure 3.
Fig. 3. Conformational changes. The superimposed -carbon
backbones of the Taq RNAP holoenzyme alone (1) and the
holoenzyme within the fork-junction DNA complex are shown as
worms (view the same as Fig. 2A). The structure of holoenzyme
alone is colored gray (core RNAP) and black ( ). The two
modules that move in the holoenzyme-DNA complex as compared with
the holoenzyme alone are colored as follows: clamp + [2],
magenta and orange (respectively); flap +
[4], blue
and orange (respectively). The phosphate backbones of the DNA in
the holoenzyme/DNA complex are shown as ribbons and colored
green (template strand, t) and light green (nontemplate strand,
nt). The downstream direction is indicated. The movements of the
mobile modules from the holoenzyme structure to their positions
in the holoenzyme-DNA complex are indicated by the arrows.
The above figure is reprinted
by permission from the AAAs:
Science
(2002,
296,
1285-1290)
copyright 2002.
-carbon
backbones of the Taq RNAP holoenzyme alone (1) and the
holoenzyme within the fork-junction DNA complex are shown as
worms (view the same as Fig. 2A). The structure of holoenzyme
alone is colored gray (core RNAP) and black ( 
). The two
modules that move in the holoenzyme-DNA complex as compared with
the holoenzyme alone are colored as follows: clamp + 
flap +