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Figure 3.
Figure 3. CBP80 Contains a Repeated Six-Helix Motif and a
MIF4G Domain(A) Repeats 1, 2, and 3 corresponding to the
N-terminal six helices of the CBP80 domains 1, 2, and 3.
Structurally homologous domains identified by the program DALI
are shown in the same orientation: the ENTH domain from Epsin 1
(Hyman et al., 2000) and the VHS domain from TOM1 (Misra et al.,
2000). The face formed by the A1, A2, and A3 helices corresponds
to the concave face and that formed by the B1, B2 and B3
helices, to the convex face. The figure was generated with
BOBSCRIPT (Esnouf, 1999).(B) Structure-based alignment of the
three six-helix motifs in CBP80 and the corresponding motif from
the MIF4G domain of eIF4GII (Marcotrigiano et al., 2001). The
homologies between repeats 1 and 2 and eIF4GII are
highlighted in red and the conserved residues among all four
structures are represented by yellow boxes. Identities are
indicated with red boxes.(C) Stereodiagram showing the
superposition of the MIF4G domains of CBP80 (residues 26–240,
blue) and eIF4GII (residues 753–986, cyan). The DALI Z score
for this superposition is 13.4. The N terminus is at the top.(D)
Structure-based alignment of the MIF4G domains of CBP80 and
eIF4GII. Identities are indicated by red boxes, and conservative
substitutions by yellow boxes. Note that eIF4GII has a long
linker between helices A3 and B3 (disordered in the crystal
structure), whereas CBP80 has a long linker between A5 and B5
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