Figure 3.
Fig. 3. Phosphate group recognition by arginines. (A) The H
resonances
of Arg1369 and Arg1374 shift downfield upon PtdIns(3)P
interaction (lower panel), indicating the formation of new
hydrogen bonds. The H[2]N resonances
of the Arg1370 and Arg1374 become resolved only in the presence
of PtdIns(3)P (upper panel), indicating that the guanidino group
rotation is impeded by PtdIns(3)P coordination. Two regions of
five 1H-15N correlation spectra of the FYVE domain (1 mM) with
PtdIns(3)P concentrations of 0, 0.1, 0.5, 1, and 5 mM are
superimposed. Arginine's structure is shown in the upper right.
(B) PtdIns(3)P moieties recognized by the FYVE domain are
indicated by absolute changes in the 1H, 13C, and 31P chemical
shifts of PtdIns(3)P induced by addition of equimolar FYVE
domain. Atoms are numbered according to the positions indicated
in (C). (C) Model of dibutanoyl PtdIns(3)P, with atom colors
based on chemical shift perturbations induced by addition of
equimolar FYVE domain, as indicated in (B).
The above figure is reprinted
by permission from the AAAs:
Science
(2001,
291,
1793-1796)
copyright 2001.
resonances
of Arg1369 and Arg1374 shift downfield upon PtdIns(3)P
interaction (lower panel), indicating the formation of new
hydrogen bonds. The H[2]N 
resonances
of the Arg1370 and Arg1374 become resolved only in the presence
of PtdIns(3)P (upper panel), indicating that the guanidino group
rotation is impeded by PtdIns(3)P coordination. Two regions of
five 1H-15N correlation spectra of the FYVE domain (1 mM) with
PtdIns(3)P concentrations of 0, 0.1, 0.5, 1, and 5 mM are
superimposed. Arginine's structure is shown in the upper right.
(B) PtdIns(3)P moieties recognized by the FYVE domain are
indicated by absolute changes in the 1H, 13C, and 31P chemical
shifts of PtdIns(3)P induced by addition of equimolar FYVE
domain. Atoms are numbered according to the positions indicated
in (C). (C) Model of dibutanoyl PtdIns(3)P, with atom colors
based on chemical shift perturbations induced by addition of
equimolar FYVE domain, as indicated in (B).