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Figure 3.
Figure 3. Structural comparison of NMR and crystallographic
LA-2 epitope maps. (a) Schematic of OspA. The view is nearly
"end-on", with the C-terminal domain of the molecule in the
foreground. The polypeptide backbone is shown as a worm tube
with labeled N and C-terminal ends. The three contiguous regions
recognized by LA-2 are identified by blue, green, and magenta
shading, respectively, and labeled by loop number as defined in
the main text. The positions of Ala208 and Ala215 are indicated
by red C^a-C^b bonds. Strictly conserved Trp216 on b-strand 17
is shown in cyan for reference. (b) By-residue
NMR-chemical-shift perturbation data mapped onto the OspA
structure, with non-hydrogen atoms shown as spheres (color
scheme as in Figure 2). (c) Crystallographic identification of
the LA-2 epitope. OspA atoms are shaded using a color gradient
by their minimum distance from LA-2 atoms. Red, <3.5 Å;
orange-bright yellow, 3.5-7.0 Å; yellow-faint yellow,
7.0-10 Å; white/gray, >10 Å. In (b) and (c), the C^b
atoms of Ala208 and Ala215 are indicated with asterisks.
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