Figure 3 - full size

 

Figure 3.
Figure 3 (A) Superposition of all seven blades of Tup1. The C[ ]backbone for each of the seven blades was aligned with respect to strands 3B and 3C. The four conserved side chains involved in the intrablade hydrogen-bonding network, referred to as the structural tetrad, are shown for blade 3 as ball-and-stick models colored according to atom type and hydrogen bonds are indicated with dashed lines. A representative blade from G , blade 4, is shown for reference. Blade 1, light green; blade 2, red; blade 3, green; blade 4, purple; blade 5, pink; blade 6, cyan; blade 7, orange; G , brown. (B) Superposition of the WD40 propellers. The C[ ]backbones for G (blue; 1TBG) and Tup1 (yellow), which align with an r.m.s.d. of 1.3 Å, are shown in the same view as Figure 1A and labeled. Least-squares alignments for (A) and (B) were done in O (Jones et al., 1991) and figures generated with RIBBONS (Carson, 1997).

The above figure is reprinted from an Open Access publication published by Macmillan Publishers Ltd: EMBO J (2000, 19, 3016-3027) copyright 2000.