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Figure 3.
Fig. 3. A broad cleft at the interface of the N and C lobes
contains highly conserved residues whose mutation reduces the
formation of the thioester or isopeptide bond. (A) The molecular
surface of the E6AP hect domain is colored according to the
conservation in 18 hect sequences: human E6AP, Nedd4, y032,
tr12, rat Urb1, Saccharomyces pombe Pub1, all five hect E3s of
Saccharomyces cerevisiae (Rsp5, Tom1, Ufd4, Hul4, and Hul5),
four hect proteins from Caenorhabditis elegans (GenBank
accession numbers BAA21847, CAA19508, CAA86773, and CAA91061),
and two Drosophila melanogaster hect proteins (the hyperplastic
disc protein and one with GenBank accession number AAD38975).
The two views are related by a rotation of ~80° about the
vertical axis. The view on the left has an orientation similar
to that of Fig. 1A; that on the right is similar to Fig. 2. The
position of the broad cleft is approximately marked by a black
line. [Prepared with the program GRASP (43).] (B) Close-up view
of the broad cleft. The N and C lobes of the hect domain are
colored red and green, respectively. The hinge region (738 to
740) between the N and C lobes is white; the conserved side
chains are yellow. The residues mutated in Angelman syndrome are
indicated with white spheres. Orientation is similar to that of
Fig. 1A.
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