|
Figure 2.
Figure 2 Titin kinase constructs used in this study. The
domain pattern of the kinase region of human titin and the
residues of the respective constructs are shown. Fn3,
fibronectin-III-like domain; Ig, immunoglobulin like domain. a,
Kin4 lacks the regulatory tail (RT), leaving a constitutively
active catalytic core. The kin1 construct includes the
regulatory tail. The N-terminal phasing is based on the N
termini of DAP kinase^31 and of Dictyostelium discoideum MLCK42.
b, Two-hybrid screens of human skeletal and cardiac
complementary DNA libraries with kin4 and kin4(K36A) yield
multiple overlapping clones from the regulatory tail, showing
that there is a functional binding site in the active site of
kin4. The strength of interaction is given by HIS3 signals and
by  -galactosidase
activity30. The first residue in the cardiac titin sequence of
three representative clones is shown at the left.
|
