|
Figure 2.
Figure 2 Three-dimensional structure of the p19^INK4d/Cdk6
complex. a, Stereo view of the C  trace
of the p19^INK4d/Cdk6 structure. Every tenth C atom
is indicated by a solid ball. b, Schematic drawing of the same
complex after rotation by 90° about the x axis. c, P19^INK4d and
p27^Kip1 prevent each other binding to the CDK subunit in cyclin
D/Cdk6. The structure in c is rotated 20° about the z axis
compared to b. In a and b p19^INK4d is coloured yellow, apart
from helix 3
(residues 46-50), which is red. The C-terminal domain of Cdk6 is
coloured light blue, whereas the N-terminal domain, which
undergoes extensive movement, is dark blue. In c, p19^INK4d is
coloured yellow, Cdk6 is light/dark blue, p27^Kip1 is red and
cyclin A is green. p27^Kip1 and cyclin A from the
p27^Kip1/cyclin A/Cdk2 structure^40 were superimposed, as
described in Fig. 5a, on p19^INK4d/Cdk6 (r.m.s. deviation was
0.46 ? over 70 residues). Figures 2 and 5a, b were produced
using the programs MOLSCRIPT47 and RASTER3D^48.
|
