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Figure 4.
Figure 4. An annealed omit electron density in the active site of RNase A. An 8 Å sphere around the sulfate ion
in the RNase active site was omitted and the immediate surrounding atoms consisting of a 3 Å shell were restrained
to avoid artificial movement into the omitted region. Simulated annealing was run with a starting temperature of
1000 Å . The stereo view of the electron density calculated with the phases derived from the omitted model and
coefficients =Fobs= - =Fcalc= for data between 40 and 2.5 Å , was contoured at 3s and plotted with a program written by
D. Xia. Superimposed is the model of the RNase A-RI complex. RNase A residues are indicated with E, and RI residues
are indicated with I.
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