|
Figure 4.
Figure 4. Detail of the Proposed Active Site of the AlkA
ProteinThe cleft, viewed along the direction of the arrow in
Figure 3D, is rich in electron-donating aromatic side chains,
which are well suited to recognize electron-deficient methylated
bases through π–donor/acceptor interactions. The
catalytically essential Asp-238 (green) lies at the bottom of
the cleft, where it is poised to participate in the reaction
chemistry, and to interact with mechanism-based oligonucleotide
inhibitors.
|
