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Figure 2.
Figure 2. Fourier maps for the unliganded N1G9 Fab crystal. (a) The 2Fo - Fc map is shown for the vicinity of Arg50H,
contoured at 1.5s level. (b) The 2Fo - Fc difference Fourier map shown for the vicinity of Trp200H, whose side-chain
N
e1
atom forms two hydrogen bonds (shown by the broken lines) with a water molecule (indicated by a cross) and with
the carbonyl O of Ile223H. For the preparation of panel b, the side-chain of Trp200H was omitted from the unliganded
structure, and then the structure was refined with X-PLOR. The phases and amplitudes thus obtained were used in
the calculation of the 2Fo - Fc difference Fourier map, contoured at 1.2s level. The Figures were produced using
Turbo-FRODO (Roussel & Cambillau, 1991). The structural models labeled with sequence numbers are superimposed
on the maps.
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