Figure 2 - full size

Figure 2. (a) Superposition of the motavizumab-bound peptide (gray) and residues 229–252 of the PIV5 F glycoprotein structure (red). (b) Ribbon representation of the model of motavizumab Fab (green and blue) bound to the PIV5 F glycoprotein monomer (tan) via the superposition shown in a. (c) Same as b, except the entire PIV5 F glycoprotein trimer is shown (tan, green, pink). (d) Magnification of the boxed area shown in c. (e) Gel filtration elution profile and corresponding Coomassie blue–stained SDS-PAGE gel of the RSV F[0] Fd glycoprotein. (f) Gel filtration elution profile and corresponding Coomassie blue–stained SDS-PAGE gel of a mixture of RSV F[0] Fd glycoprotein and excess palivizumab Fab. Densitometric analysis of the gel yields a ratio of 2.97 Fabs per trimer.

The above figure is reprinted by permission from Macmillan Publishers Ltd: Nat Struct Biol (2010, 17, 248-250) copyright 2010.