Figure 2.
Figure 2: tRNA recognition by GkTilS. a, Overall homodimeric
structure. b, Recognition of the anticodon loop. F[o ]- F[c]
simulated annealing omit maps (3.7 )
of Arg 142 and three nucleotides (C32, C34 and A38) are shown in
magenta and grey, respectively. c, Left, recognition of the
major groove of the acceptor stem by the HTH motif and -hairpin;
right, the protein surface that complementarily interacts with
the 3'-ACCA terminus is coloured according to its electrostatic
potential. d, Lysidine incorporating activities of tRNA^Ile2 and
GkTilS mutants. The initial rate of lysidine incorporation is
shown. Error bars, s.d. of three independent experiments.
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
Nature
(2009,
461,
1144-1148)
copyright 2009.
)
of Arg 142 and three nucleotides (C32, C34 and A38) are shown in
magenta and grey, respectively. c, Left, recognition of the
major groove of the acceptor stem by the HTH motif and 
-hairpin;
right, the protein surface that complementarily interacts with
the 3'-ACCA terminus is coloured according to its electrostatic
potential. d, Lysidine incorporating activities of tRNA^Ile2 and
GkTilS mutants. The initial rate of lysidine incorporation is
shown. Error bars, s.d. of three independent experiments.